Minimum Inhibition Concentration of surface-modified ZnO nanostructures on Streptococcus mutans

Abstract In this study, minimum inhibition concentration (MIC) and bactericidal (MBC) were estimated for three types of zinc oxide (ZnO) nanoparticles on a Gram-positive bacterial species: Streptococcus mutans . The structural properties these nanoparticles, designated as ZnO-A, ZnO-K, ZnO-Ax, characterized using the techniques field-emission scanning electron microscopy, X-ray diffraction, photoluminescence spectroscopy. Both MIC MBC evaluated series dilutions (serial dilution) in 96-microtiter plate following standard method CLSI M100-Ed32. commercial ZnO-K nanoparticle had largest average crystallite size, i.e., 42 nm followed by ZnO-A (37.5 nm), ZnO-Ax (37.8 nm). was synthesized via post-oxygen annealing, while prepared energy combustion. All ZnO yielded similar value, 0.156 mM, S. However, colony-forming unit (CFU/mL) at mM varied among particles. lowest colony number mutans, suggesting that gave better towards bacteria, probably due to its high surface area O: Zn ratio (1.09) enhanced generation reactive oxygen species antibacterial activity compared ZnO-K.